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Background free imaging of single mRNAs in live cells using split fluorescent proteins.
- Source :
-
Scientific Reports . 1/10/2014, p1-3. 3p. - Publication Year :
- 2014
-
Abstract
- We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLUORESCENT proteins
*NUCLEIC acids
*RIBOSE
*MESSENGER RNA
*COAT proteins (Viruses)
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- Academic Search Index
- Journal :
- Scientific Reports
- Publication Type :
- Academic Journal
- Accession number :
- 94518273
- Full Text :
- https://doi.org/10.1038/srep03615