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Cathelicidin Antimicrobial Peptide LL-37 in Cholesteatoma Enables Keratinocyte Reactivity with Cytosolic DNA.
- Source :
-
Scandinavian Journal of Immunology . Mar2014, Vol. 79 Issue 3, p214-221. 8p. - Publication Year :
- 2014
-
Abstract
- The purpose of this study was to determine whether self- DNA can trigger the inflammatory response in cholesteatoma. Specimens were collected from nine patients with invasive cholesteatoma, nine patients with attic-type cholesteatoma (pars flaccida was perforated in five patients and intact in four) and four healthy skins. Expression and localization of LL-37 and interferon-alpha were detected by immunofluorescence and immunoblot analysis. Cultures of human cholesteatomatous keratinocytes were exposed to CpG DNA, LL-37 or CpG DNA complexed to LL-37 for 24 h. Expression of interferon-alpha was detected by RT- PCR. We detected abundant cytosolic DNA, increased LL-37 and interferon-alpha in keratinocytes in invasive cholesteatoma and attic-type cholesteatoma with pars flaccida perforation, but not in attic-type cholesteatoma with pars flaccida intact and normal skin. In cultured keratinocytes, LL-37- DNA complexes induced IFN- α expression. These data suggest that cytosolic DNA is an important disease-associated molecular pattern that triggers the inflammation response in cholesteatoma. Furthermore, LL-37 played an important role in DNA-triggered inflammation. Thus, we have identified a link between cytosolic DNA, LL-37 and autoinflammation in cholesteatoma, providing new potential targets for the treatment of this disease. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03009475
- Volume :
- 79
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Scandinavian Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 94630777
- Full Text :
- https://doi.org/10.1111/sji.12149