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Evolutionarily Conserved Requirement for Core Binding Factor Beta in the Assembly of the Human Immunodeficiency Virus/Simian Immunodeficiency Virus Vif-Cullin 5-RING E3 Ubiquitin Ligase.
- Source :
-
Journal of Virology . Mar2014, Vol. 88 Issue 6, p3320-3328. 9p. - Publication Year :
- 2014
-
Abstract
- The human immunodeficiency virus type 1 (HIV-1)-encoded virion infectivity factor (Vif) is required to inactivate the host restriction factor APOBEC3 by engaging Cullin 5 (Cul5)-RING ubiquitin ligase (CRL5). Core binding factor beta (CBF-β) is a novel regulator of Vif-CRL5 function; as yet, its mechanism of regulation remains unclear. In the present study, we demonstrate that CBF-β promotion of Vif-CRL5 assembly is independent of its influence on Vif stability and is also a conserved feature of primate lentiviral Vif proteins. Furthermore, CBF-β is critical for the formation of the Vif-ElonginB/ElonginC-Cul5 core E3 ubiquitin ligase complex in vitro. CBF-β from diverse vertebrate species supported HIV-1 Vif function, indicating the conserved nature of Vif-CBF-β interfaces. Considering the importance of the interaction between Vif and CBF-β in viral CRL5 function, disrupting this interaction represents an attractive pharmacological intervention against HIV-1. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HIV
*SIMIAN immunodeficiency virus diseases
*VIRION
*UBIQUITIN ligases
*ELONGINS
Subjects
Details
- Language :
- English
- ISSN :
- 0022538X
- Volume :
- 88
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Virology
- Publication Type :
- Academic Journal
- Accession number :
- 94739819
- Full Text :
- https://doi.org/10.1128/JVI.03833-13