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Quantification analysis of yeast-displayed lipase.
- Source :
-
Analytical Biochemistry . Apr2014, Vol. 450, p46-48. 3p. - Publication Year :
- 2014
-
Abstract
- Abstract: We describe a method for quantification of displayed lipase on yeast cell surface. The strategy uses an organophosphonate ester to irreversibly inhibit the active lipase and release a detectable fluorescent group. The amount of displayed lipase can be represented as “g/g cell” or “molecules/cell”. The results obtained correlated well with those obtained by existing methods. Therefore, this method is credible and will provide a powerful tool to promote research of lipase yeast surface display. [Copyright &y& Elsevier]
- Subjects :
- *YEAST
*LIPASES
*FLUORESCENT proteins
*PHOSPHONATES
*ESTERS
Subjects
Details
- Language :
- English
- ISSN :
- 00032697
- Volume :
- 450
- Database :
- Academic Search Index
- Journal :
- Analytical Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 94756546
- Full Text :
- https://doi.org/10.1016/j.ab.2013.12.035