Back to Search
Start Over
Multiplexed label-free optical biosensor for medical diagnostics.
- Source :
-
Journal of Biomedical Optics . Jan2014, Vol. 19 Issue 1, p1-10. 10p. - Publication Year :
- 2014
-
Abstract
- This paper describes a new multiplexed label-free biosensor. The detection technology is based on nanostructured gold-polymer surfaces. These surfaces support surface plasmon resonance modes that can be probed by a miniaturized optical setup. The optical characterization of the sensing chip shows the sensitivity and the limit-of-detection to refractive index changes. Moreover, by studying the progressive adhesion of molecular monolayers of polyelectrolytes, the decay of the plasmonicmode electric field above the surface has been recon- structed. A multiplexed label-free biosensing device is then described and characterized in terms of sensitivity, lateral resolution, and sensitivity to a model biological assay. The sensitivity in imaging mode of the device is of the order of 10-6 refractive index units, while the measured lateral resolution is 6.25 µm within a field of view of several tenths of mm², making the instrument unique in terms of multiplexing capability. Finally, the proof-of-concept application of the technology as a point-of-care diagnostic tool for an inflammatory marker is demonstrated. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BIOSENSOR research
*POLYMERS
*GOLD
*SURFACE plasmon resonance
*POLYELECTROLYTES
Subjects
Details
- Language :
- English
- ISSN :
- 10833668
- Volume :
- 19
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Biomedical Optics
- Publication Type :
- Academic Journal
- Accession number :
- 94830135
- Full Text :
- https://doi.org/10.1117/1.JBO.19.1.017006