Development of a varicella-zoster virus neutralization assay using a glycoprotein K antibody enzyme-linked immunosorbent spot assay.

Highlights: [•] Similar to the ELISPOT method, we developed a VZV neutralization enzyme-linked immunosorbent spot (N-ELISPOT) system. [•] We applied it to quantify virus, optimizing the test antibody dilution. [•] Compared with the plaque assay, the VZV N-ELISPOT was accurate and reliable for the detection of neutralizing serum samples. [ABSTRACT FROM AUTHOR]