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AtomicForce Microscopy Probing of Receptor–NanoparticleInteractions for Riboflavin Receptor Targeted Gold–DendrimerNanocomposites.
- Source :
-
Journal of Physical Chemistry B . Mar2014, Vol. 118 Issue 11, p2872-2882. 11p. - Publication Year :
- 2014
-
Abstract
- Riboflavin receptors are overexpressedin malignant cells fromcertain human breast and prostate cancers, and they constitute a groupof potential surface markers important for cancer targeted deliveryof therapeutic agents and imaging molecules. Here we report on thefabrication and atomic force microscopy (AFM) characterization ofa core–shell nanocomposite consisting of a gold nanoparticle(AuNP) coated with riboflavin receptor-targeting poly(amido amine)dendrimer. We designed this nanocomposite for potential applicationssuch as a cancer targeted imaging material based on its surface plasmonresonance properties conferred by AuNP. We employed AFM as a techniquefor probing the binding interaction between the nanocomposite andriboflavin binding protein (RfBP) in solution. AFM enabled precisemeasurement of the AuNP height distribution before (13.5 nm) and afterchemisorption of riboflavin-conjugated dendrimer (AuNP–dendrimer;20.5 nm). Binding of RfBP to the AuNP–dendrimer caused a heightincrease to 26.7 nm, which decreased to 22.8 nm when coincubated withriboflavin as a competitive ligand, supporting interaction of AuNP–dendrimerand its target protein. In summary, physical determination of sizedistribution by AFM imaging can serve as a quantitative approach tomonitor and characterize the nanoscale interaction between a dendrimer-coveredAuNP and target protein molecules in vitro. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15206106
- Volume :
- 118
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Journal of Physical Chemistry B
- Publication Type :
- Academic Journal
- Accession number :
- 95055499
- Full Text :
- https://doi.org/10.1021/jp412053w