Rapid and sensitive detection of the inhibitive activities of acetyl- and butyryl-cholinesterases inhibitors by UPLC–ESI-MS/MS.

Highlights: [•] UPLC–ESI MS/MS method for detection of AChE and BChE inhibition. [•] The method is successfully applied to detect inhibitive activities of model drugs. [•] The assay is run within 1.2min with acceptable sensitivity and reproducibility. [•] The method is economical for substrate and enzyme consumption. [•] The method is suitable for screening AChE and BChE inhibitor from natural product. [Copyright &y& Elsevier]