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Technical note: Optimization of lactose quantification based on coupled enzymatic reactions.
- Source :
-
Journal of Dairy Science . Apr2014, Vol. 97 Issue 4, p2066-2070. 5p. - Publication Year :
- 2014
-
Abstract
- A colorimetric microplate-adapted lactose assay was developed to quantify lactose in dairy products. The assay was based on the coupled enzymatic reaction of β-galactosidase-glucose oxidase-horseradish peroxidase using Amplex red as detection probe. The assay showed good linearity in the range of 0.1 to 0.5 mmol of lactose/L, with a limit of detection of 0.0433 mmol/L and a limit of quantification of 0.1313 mmol/L. The lactose assay at optimized conditions (5 U of β-galactosidase/ mL, 5 U of glucose oxidase/mL, 1 U of horseradish peroxidase/mL, and 100 µmol of Amplex red/L for 1 h at 37°C in the dark) showed good correlation with a commercial lactose enzymatic kit with intraassay variation below 10% and interassay variations below 7.6%. The developed lactose microplate assay can be adopted as routine analysis for lactose determination in dairy products due to its relatively low cost compared with a commercial kit, relatively short reaction time, and high sensitivity and reproducibility. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DAIRY products
*LACTOSE
*GALACTOSIDASES
*HORSERADISH
*PEROXIDASE
Subjects
Details
- Language :
- English
- ISSN :
- 00220302
- Volume :
- 97
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Dairy Science
- Publication Type :
- Academic Journal
- Accession number :
- 95256586
- Full Text :
- https://doi.org/10.3168/jds.2013-7436