Combination of aqueous two-phase extraction and cation-exchange chromatography: New strategies for separation and purification of alliin from garlic powder.

Highlights: [•] ATPS was first used as a solvent to extract alliin from garlic powder. [•] Compared with traditional extraction method, aqueous two-phase extraction (ATPE) not only improved the alliin yield but also concentrated extract solution of alliin to a large extent. [•] Chromatography was used following by ATPE to purify alliin, the combination of them provided a rapid and effective method for alliin extraction and purification. [•] In this paper, cation-exchange resins 001Ã?7 was converted to NH4 + form. [ABSTRACT FROM AUTHOR]