Methodological considerations for the harmonization of non-cholesterol sterol bio-analysis.

Highlights: [•] Non-cholesterol sterols (NCS) are used as surrogate markers of cholesterol metabolism. [•] NCS analysis methodology is highly variable across different research groups. [•] Variability in NCS methodology hampers comparisons across research groups. [•] We examined the critical steps in the extraction, analysis and reporting of NCS. [•] A cross-comparison program is needed for the standardization of NCS analysis. [ABSTRACT FROM AUTHOR]