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Plasmid DNA encoding human carcinoembryonic antigen (CEA) adsorbed onto cationic microparticles induces protective immunity against colon cancer in CEA-transgenic mice
- Source :
-
Vaccine . May2003, Vol. 21 Issue 17/18, p1938. 10p. - Publication Year :
- 2003
-
Abstract
- A carcinoembryonic antigen (CEA)-based DNA vaccine, adsorbed onto cationic microparticles of poly(dl-lactide-co-glycolide) (PLG) induced tumor-protective immunity against a lethal challenge of MC38-CEA colon carcinoma cells in CEA-transgenic mice that was more potent than that of the corresponding naked DNA vaccine. Boosting with a plasmid encoding murine GM-CSF increased the vaccine’s efficacy leading to a complete rejection of tumor cells in 50% of mice. This effect was due to activation of MHC class I-restricted CD8+ T cells coupled with an increased secretion of proinflammatory cytokines IFN-γ, TNF-α and IL-2. Also, specific activation of dendritic cells was indicated by a two–three-fold upregulation of their costimulatory CD80 and MHC class II molecules. This approach may be a promising new strategy for the rational design of cancer vaccines for future clinical applications. [Copyright &y& Elsevier]
- Subjects :
- *DNA vaccines
*GREEN fluorescent protein
Subjects
Details
- Language :
- English
- ISSN :
- 0264410X
- Volume :
- 21
- Issue :
- 17/18
- Database :
- Academic Search Index
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 9544460
- Full Text :
- https://doi.org/10.1016/S0264-410X(02)00821-6