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Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis.
- Source :
-
Evidence-based Complementary & Alternative Medicine (eCAM) . 2013, Vol. 2013, p1-15. 15p. 2 Charts, 5 Graphs. - Publication Year :
- 2013
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Abstract
- This study investigated the effect of Angelica sinensis polysaccharides (APS-3c) on rat osteoarthritis (OA) model in vivo and rat interleukin-1-beta- (IL-1β-) stimulated chondrocytes in vitro. APS-3c was administrated into rat OA knee joints and had protective effects on rat OA cartilage in vivo. Primary rat articular chondrocytes were cotreated with APS-3c and IL-1β in vitro. 2-50 μg/mL APS-3c had no effect on chondrocytes viability, whereas it increased the proteoglycans (PGs) synthesis inhibited by IL-1β. Microarray analysis showed that the significant changes were concentrated in the genes which were involved in PGs synthesis. RT-PCR confirmed that treatment with APS-3c increased the mRNA expression of aggrecan and glycosyltransferases (GTs) inhibited by IL-1β but did not affect the mRNA expression of matrix-degrading enzymes. These results indicate that APS-3c can improve PGs synthesis of chondrocytes on rat OA model in vivo and IL-lβ-stimulated chondrocytes in vitro, which is due to the promotion of the expression of aggrecan and GTs involved in PGs synthesis but not the inhibition of the expression of matrix-degrading enzymes. Our findings suggest the clinical relevance of APS-3c in the prospective of future alternative medical treatment for OA. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANALYSIS of variance
*ANIMAL experimentation
*BIOPHYSICS
*CARTILAGE
*FLOW cytometry
*GLYCOSAMINOGLYCANS
*KNEE diseases
*MARINE animals
*RESEARCH methodology
*OSTEOARTHRITIS
*HEALTH outcome assessment
*POLYMERASE chain reaction
*POLYSACCHARIDES
*RATS
*RESEARCH funding
*WESTERN immunoblotting
*TREATMENT effectiveness
*REVERSE transcriptase polymerase chain reaction
*DONG quai
*DATA analysis software
*GENE expression profiling
*IN vitro studies
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Volume :
- 2013
- Database :
- Academic Search Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 95495411
- Full Text :
- https://doi.org/10.1155/2013/794761