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Testing of potential glycan-based heparanase inhibitors in a fluorescence activity assay using either bacterial heparinase II or human heparanase.
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . Jul2014, Vol. 95, p130-138. 9p. - Publication Year :
- 2014
-
Abstract
- Highlights: [•] A simple fluorimetric heparanase activity assay using the synthetic sulfated pentasaccharide fondaparinux as substrate. [•] The assay was used for testing potential heparanase inhibitors. [•] The inhibitory activity of sulfated glycans showed to be strongly dependent on their degree of sulfation as well as their basic glycan structure, but independent of their molecular mass. [•] The semisynthetic β-1,3-glucan sulfate PS3 showed the strongest inhibitory effect with an IC50 being 160 times lower than that of suramin. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 95
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 95504320
- Full Text :
- https://doi.org/10.1016/j.jpba.2014.02.021