Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging.

Highlights: [•] Staining protocols were optimized to study myofibrillogenesis in Xenopus embryos. [•] A dissection approach was developed for ideal staining of Xenopus somitic regions. [•] Optimized phalloidin labeling and immunostaining for Xenopus embryo cryosections. [•] Demonstrated methods to preserve phalloidin labeling after Murray’s clear. [Copyright &y& Elsevier]