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Expression and characterization of a GH43 endo-arabinanase from Thermotoga thermarum.
- Source :
-
BMC Biotechnology . 2014, Vol. 14 Issue 1, p1-20. 20p. - Publication Year :
- 2014
-
Abstract
- Background Arabinan is an important plant polysaccharide degraded mainly by two hydrolytic enzymes, endo-arabinanase and α-L-arabinofuranosidase. In this study, the characterization and application in arabinan degradation of an endo-arabinanase from Thermotoga thermarum were investigated. Results The recombinant endo-arabinanase was expressed in Escherichia coli BL21 (DE3) and purified by heat treatment followed by purification on a nickel affinity column chromatography. The purified endo-arabinanase exhibited optimal activity at pH 6.5 and 75°C and its residual activity retained more than 80% of its initial activity after being incubated at 80°C for 2 h. The results showed that the endo-arabinanase was very effective for arabinan degradation at higher temperature. When linear arabinan was used as the substrate, the apparent Km and Vmax values were determined to be 12.3 ± 0.15 mg ml-1 and 1,052.1 ± 12.7 μmol ml-1 min-1, respectively (at pH 6.5, 75°C), and the calculated kcat value was 349.3 ± 4.2 s-1. Conclusions This work provides a useful endo-arabinanase with high thermostability andcatalytic efficiency, and these characteristics exhibit a great potential for enzymatic conversion of arabinan. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14726750
- Volume :
- 14
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- BMC Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 96046830
- Full Text :
- https://doi.org/10.1186/1472-6750-14-35