P57: The expression of cystathionine-β-synthase in the brain of spontaneous hypertensive rat.

Objective: To investigate the distribution of cystathionine-beta-synthase (CBS) in the brain of the spontaneous hypertensive rat. Method: Eight adult male spontaneously hypertensive rats (SHR), and 8 pargue-Darley (SD) rats were assigned into hypertensive group and control group correspondingly. All rats were killed by over-dose anesthesia. The cerebral cortex, cerebellum, hypothalamus, hippocampus, medulla oblongata were dissociated quickly. Their mRNA of CBS was detected by RT-PCR or Real Time-PCR. Results: The transcriptional levels of CBS in brains in the SHR group were significantly decrease compared with the normal control group (0.93±0.01 vs. 1.00±0.00, P<0.05; 0.39±0.02 vs. 1.00±0.00, P<0.01; 0.07±0.01 vs. 1.00±0.00, P<0.01; 0.09±0.03 vs. 1.00±0.00, P<0.01; 0.36±0.06 vs. 1.00±0.00, P<0.01). Conclusion: The expression of CBS mRNA in brain tissue of SHR rats are down-regulated, it is likely to get through the mechanism of central, such as medulla oblongata, which probably involved in the pathogenesis of hypertension. [ABSTRACT FROM AUTHOR]