Back to Search Start Over

Orientation of llama antibodies strongly increases sensitivity of biosensors.

Authors :
Trilling, Anke K.
Hesselink, Thamara
Houwelingen, Adèle van
Cordewener, Jan H.G.
Jongsma, Maarten A.
Schoffelen, Sanne
Hest, Jan C.M. van
Zuilhof, Han
Beekwilder, Jules
Source :
Biosensors & Bioelectronics. Oct2014, Vol. 60, p130-136. 7p.
Publication Year :
2014

Abstract

Abstract: Sensitivity of biosensors depends on the orientation of bio-receptors on the sensor surface. The objective of this study was to organize bio-receptors on surfaces in a way that their analyte binding site is exposed to the analyte solution. VHH proteins recognizing foot-and-mouth disease virus (FMDV) were used for making biosensors, and azides were introduced in the VHH to function as bioorthogonal reactive groups. The importance of the orientation of bio-receptors was addressed by comparing sensors with randomly oriented VHH (with multiple exposed azide groups) to sensors with uniformly oriented VHH (with only a single azide group). A surface plasmon resonance (SPR) chip exposing cyclooctyne was reacted to azide functionalized VHH domains, using click chemistry. Comparison between randomly and uniformly oriented bio-receptors showed up to 800-fold increase in biosensor sensitivity. This technique may increase the containment of infectious diseases such as FMDV as its strongly enhanced sensitivity may facilitate early diagnostics. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
09565663
Volume :
60
Database :
Academic Search Index
Journal :
Biosensors & Bioelectronics
Publication Type :
Academic Journal
Accession number :
96243767
Full Text :
https://doi.org/10.1016/j.bios.2014.04.017