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Orientation of llama antibodies strongly increases sensitivity of biosensors.
- Source :
-
Biosensors & Bioelectronics . Oct2014, Vol. 60, p130-136. 7p. - Publication Year :
- 2014
-
Abstract
- Abstract: Sensitivity of biosensors depends on the orientation of bio-receptors on the sensor surface. The objective of this study was to organize bio-receptors on surfaces in a way that their analyte binding site is exposed to the analyte solution. VHH proteins recognizing foot-and-mouth disease virus (FMDV) were used for making biosensors, and azides were introduced in the VHH to function as bioorthogonal reactive groups. The importance of the orientation of bio-receptors was addressed by comparing sensors with randomly oriented VHH (with multiple exposed azide groups) to sensors with uniformly oriented VHH (with only a single azide group). A surface plasmon resonance (SPR) chip exposing cyclooctyne was reacted to azide functionalized VHH domains, using click chemistry. Comparison between randomly and uniformly oriented bio-receptors showed up to 800-fold increase in biosensor sensitivity. This technique may increase the containment of infectious diseases such as FMDV as its strongly enhanced sensitivity may facilitate early diagnostics. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 60
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 96243767
- Full Text :
- https://doi.org/10.1016/j.bios.2014.04.017