Back to Search
Start Over
PpIX induces mitochondria-related apoptosis in murine leukemia L1210 cells.
- Source :
-
Drug & Chemical Toxicology . Jul2014, Vol. 37 Issue 3, p348-356. 9p. - Publication Year :
- 2014
-
Abstract
- Context: Protoporphyrin IX (PpIX), a well-known sensitizer that can enhance laser light or ultrasound induced cytotoxicity in photodynamic and sonodynamic therapy. However, PpIX alone could effectively cause anti-tumor effect and the underlying mechanisms are rarely been reported. Therefore, this study was to investigate the possible mechanism by which PpIX revealed anti-proliferative effect on murine leukemia L1210 cells. Materials and methods: The accumulation of PpIX in L1210 cells and normal peripheral blood mononuclear cells (PBMCs) was evaluated with flow cytometry. The subcellular localization of PpIX and apoptosis-inducing factor (AIF) translocation were determined by confocal microscope. The cell viability was examined by MTT assay. Annexin V-PE/7-AAD and DAPI staining were used to detect apoptotic cells. The mitochondrial membrane potential (MMP) changes were tested by rhodamine123 staining. DNA damage was measured by comet assay. Results: PpIX preferentially accumulated in L1210 cells compared to PBMCs and PpIX mainly located in the mitochondria of L1210 cells. PpIX at a concentration of 1 µg/ml or above exerted significant anti-tumor effect and the cell viability loss presented PpIX dose-dependent manner. Typical apoptotic features such as chromatin condensation were observed by DAPI staining. Annexin V-PE/7-AAD analysis showed 5 µg/ml PpIX could induce about 24% cell apoptosis, which was inhibited by cyclosporin A (CsA), an inhibitor of mitochondrial permeability transition pore. In addition, the PpIX caused MMP loss, AIF translocation to nucleus and serious DNA damage were also suppressed by CsA. Conclusion: The results indicate mitochondria-dependent apoptosis were involved in PpIX caused cell damage on L1210 cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01480545
- Volume :
- 37
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Drug & Chemical Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 96253097
- Full Text :
- https://doi.org/10.3109/01480545.2013.866135