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Colorimetric protease assay by using gold nanoparticles and oligopeptides.
- Source :
-
Sensors & Actuators B: Chemical . Oct2014, Vol. 201, p234-239. 6p. - Publication Year :
- 2014
-
Abstract
- Abstract: A colorimetric protease assay based on the cleavage of gold nanoparticles (AuNPs) aggregates cross-linked with oligopeptides is developed. In this assay, when oligopeptide YHPQMNPYTKAGGGC is added to a solution containing citrate-stabilized AuNPs, cysteine and lysine on the oligopeptide causes rapid aggregation of AuNPs, resulting in color change from red to blue. However, in the presence of a model protease trypsin, the oligopeptide is cleaved by trypsin into two fragments. As a result, AuNPs do not aggregate and no color change can be observed. By using this principle, trypsin can be detected with the naked eye at a concentration as low as 0.5nM. This assay can also be applied to detect trypsin inhibitors such as benzamidine hydrochloride. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 201
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 96447145
- Full Text :
- https://doi.org/10.1016/j.snb.2014.05.014