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MicroRNA-222 promotes the proliferation and migration of cervical cancer cells.
- Source :
-
Clinical & Investigative Medicine . Jun2014, Vol. 37 Issue 3, pE131-E141. 11p. 2 Color Photographs, 1 Black and White Photograph, 1 Chart, 4 Graphs. - Publication Year :
- 2014
-
Abstract
- Purpose: This study aimed to investigate the role of small non-coding RNA-222 (microRNA-222; miR-222) in the development of cervical cancer (CC). Methods: Normal and CC specimens were obtained from 18 patients. HeLa and SiHa cells were grown in Dulbecco's modified Eagle's medium. RT-PCR, Western blot, migration assay, flow cytometry and immunofluorescence microscopy were used for analyses. Results: When compared with normal cervical tissues, miR-222 was upregulated in human CC, and the extent of up-regulation was associated with the extent and depth of CC invasion. Expression of miR-222 was inversely related to the expression of phosphatase and tensin homolog (PTEN) and p27. The reduced the expression of PTEN and p27 by miR-222 in HeLa cells and SiHa cells was associated with increased proliferation and migration of CC cells. The expression of proteins (E-cadherin and paxillin) related to the proliferation and migration was also elevated. Conclusion: MiR-222 plays an important role in the tumorigenesis of CC, possibly by specifically down-regulating p27Kip1 and PTEN. Our findings suggest that miR-222 may serve as a new therapeutic target in CC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0147958X
- Volume :
- 37
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Clinical & Investigative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 96696167
- Full Text :
- https://doi.org/10.25011/cim.v37i3.21380