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G-quadruplex DNA biosensor for sensitive visible detection of genetically modified food.
- Source :
-
Talanta . Oct2014, Vol. 128, p445-449. 5p. - Publication Year :
- 2014
-
Abstract
- In this paper, a novel label-free G-quadruplex DNAzyme sensor has been proposed for colorimetric identification of GMO using CaMV 35S promoter sequence as the target. The binary probes can fold into G-quadruplex structure in the presence of DNA-T (Target DNA) and then combine with hemin to form a DNAzyme resembling horseradish peroxidase. The detection system consists of two G-rich probes with 2:2 split mode by using the absorbance and color of ABTS2- as signal reporter. Upon the addition of a target sequence, two probes both hybridize with target and then their G-rich sequences combine to form a G-quadruplex DNAzyme, and the DNAzyme can catalyze the reaction of ABTS2- with H2O2. Then the linear range is from 0.05 to 0.5 μM while detection limit is 5 nM. These results demonstrate that the proposed G-quadruplex DNAzyme method could be used as a simple, sensitive and cost-effective approach for assays of GMO. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BIOSENSORS
*HORSERADISH
*DEOXYRIBOZYMES
*COST effectiveness
*TRANSGENIC organisms
Subjects
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 128
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 97203892
- Full Text :
- https://doi.org/10.1016/j.talanta.2014.05.002