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Characterising the atypical 5′-CG DNA sequence specificity of 9-aminoacridine carboxamide Pt complexes.
- Source :
-
Journal of Biological Inorganic Chemistry (JBIC) . Aug2014, Vol. 19 Issue 6, p997-1007. 11p. - Publication Year :
- 2014
-
Abstract
- In this study, the DNA sequence specificity of four DNA-targeted 9-aminoacridine carboxamide Pt complexes was compared with cisplatin, using two specially constructed plasmid templates. One plasmid contained 5′-CG and 5′-GA insert sequences while the other plasmid contained a G-rich transferrin receptor gene promoter insert sequence. The damage profiles of each compound on the different DNA templates were quantified via a polymerase stop assay with fluorescently labelled primers and capillary electrophoresis. With the plasmid that contained 5′-CG and 5′-GA dinucleotides, the four 9-aminoacridine carboxamide Pt complexes produced distinctly different damage profiles as compared with cisplatin. These 9-aminoacridine complexes had greatly increased levels of DNA damage at CG and GA dinucleotides as compared with cisplatin. It was shown that the presence of a CG or GA dinucleotide was sufficient to reveal the altered DNA sequence selectivity of the 9-aminoacridine carboxamide Pt analogues. The DNA sequence specificity of the Pt complexes was also found to be similarly altered utilising the transferrin receptor DNA sequence. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ACRIDINE
*CARBOXAMIDES
*PLATINUM compounds
*METAL complexes
*CISPLATIN
*DNA analysis
Subjects
Details
- Language :
- English
- ISSN :
- 09498257
- Volume :
- 19
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Inorganic Chemistry (JBIC)
- Publication Type :
- Academic Journal
- Accession number :
- 97287869
- Full Text :
- https://doi.org/10.1007/s00775-014-1144-3