Back to Search
Start Over
Synthesis of Water-Dispersed Ferrecene/Phenylboronic Acid-Modified Bifunctional Gold Nanoparticles and the Application in Biosensing.
- Source :
-
Materials (1996-1944) . Aug2014, Vol. 7 Issue 8, p5554-5564. 11p. 1 Diagram, 4 Graphs. - Publication Year :
- 2014
-
Abstract
- Phenylboronic acids can form tight covalent bonds with diol-containing biomolecules. In this work, water-dispersed bifunctional gold nanoparticles (AuNPs) modified with ferrecene (Fc)-derivatized peptides and 4-mercaptophenylboronic acids (MBA) (denoted as Fc--MBA--AuNPs) were synthesized and characterized by UV/vis spectrometry and transmission electron microscopy. To demonstrate the application and the analytical merits of the nanoparticles in biosensing, glycoprotein avidin was tested as a model analyte. Specifically, avidin was captured by the biotin-covered gold electrode via the strong biotin-avidin interaction. Then, Fc--MBA--AuNPs were attached by the captured avidin through the formation of tight covalent bonds between the boronic acid moieties of Fc--MBA--AuNPs and the oligosaccharides of avidin. As a result, a detection limit of 0.2 pM was achieved. We believe that the bifunctional nanoparticles would found many applications in amplified detection of diol-containing species by rational design of the surface chemistry of electrode. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19961944
- Volume :
- 7
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Materials (1996-1944)
- Publication Type :
- Academic Journal
- Accession number :
- 97747021
- Full Text :
- https://doi.org/10.3390/ma7085554