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Application and recovery of ionic liquids in the preparative separation of four flavonoids from Rhodiola rosea by on-line three-dimensional liquid chromatography.
- Source :
-
Journal of Separation Science . Sep2014, Vol. 37 Issue 17, p2314-2321. 8p. - Publication Year :
- 2014
-
Abstract
- A novel on-line three-dimensional liquid chromatography method was developed to separate four main flavonoids from Rhodiola rosea. Ethyl acetate/0.5 mol/L ionic liquid 1-butyl-3-methylimidazolium chloride aqueous solution was selected as the solvent system. In the first-dimension separation, the target flavonoids were entrapped and subsequently desorbed into the second-dimension high-speed countercurrent chromatographic column for separation. In the third-dimension chromatography, the residual ionic liquid in the four separated flavonoids was removed and the used ionic liquid was recovered. As a result, 35.1 mg of compound 1, 20.4 mg of compound 2, 8.5 mg of compound 3, and 10.6 mg of compound 4 were obtained from 1.53 g R. rosea extract. They were identified as rhodiosin, rhodionin, herbacetin, and kaempferol, respectively. The recovery of ionic liquid reached 99.1% of the initial amount. The results showed that this method is a powerful technology for the separation of R. rosea flavonoids and that the ionic-liquid-based solvent system has advantages over traditional solvent systems in renewable and environmentally friendly properties. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16159306
- Volume :
- 37
- Issue :
- 17
- Database :
- Academic Search Index
- Journal :
- Journal of Separation Science
- Publication Type :
- Academic Journal
- Accession number :
- 97808080
- Full Text :
- https://doi.org/10.1002/jssc.201400305