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The Antigenic Structure Characterization of Oestrus Ovis Larvae.
- Source :
-
Scientific Papers: Animal Science & Biotechnologies / Lucrari Stiintifice: Zootehnie si Biotehnologii . Jan2012, Vol. 45 Issue 1, p314-318. 5p. - Publication Year :
- 2012
-
Abstract
- With the aim of proteic components definition from Oestrus ovis larvae, endowed with antigenic properties, able to induce immune responses in vivo and to react in vitro with induced molecular effectors were been performed: electrophoresis in poliacrilamid gel, western blot technique preceded by immunotransfer, immunoassay test. Total soluble larval antigens of O. ovis were been prepared through ultrasonic disintegration, from all three larval stages. Western blot technique allowed and emphasized the specific antigens with a superior sensitivity in comparison with SDS-PAGE electrophoresis. After antigenic characteristics demonstration of investigated larval antigens were been performed the immunoassay test to emphasized the antibodies dozes for O. ovis infestation diagnosis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SHEEP botfly
*LARVAE
*ANTIGENS
*WESTERN immunoblotting
*ELECTROPHORESIS
*IMMUNOASSAY
Subjects
Details
- Language :
- English
- ISSN :
- 18419364
- Volume :
- 45
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Scientific Papers: Animal Science & Biotechnologies / Lucrari Stiintifice: Zootehnie si Biotehnologii
- Publication Type :
- Academic Journal
- Accession number :
- 98136454