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Comparison of Efficiency of Self-renewal and Differentiation Potential in Tendon-derived Mesenchymal Stem Cells Isolated by Magneticactivated Cell Sorting Method or Colony Picking Method.

Comparison of Efficiency of Self-renewal and Differentiation Potential in Tendon-derived Mesenchymal Stem Cells Isolated by Magneticactivated Cell Sorting Method or Colony Picking Method.

Authors :
Moses Lee
Yoorim Choi
Dong Suk Yoon
Jin Woo Lee
Gil Sung Yoon
Woo Jin Choi
Seung Hwan Han
Source :
Journal of Korean Foot & Ankle Society. Sep2014, Vol. 18 Issue 3, p100-107. 8p.
Publication Year :
2014

Abstract

Purpose: The purpose of this study is to evaluate the efficacy of mesenchymal stem cell (MSC) isolation by the magnetic-activated cell sorting (MACS) method in tendon tissue-derived cells compared to the colony picking method for isolation of MSCs by picking colonyforming cells. Materials and Methods: Human tendon-derived cells were isolated by enzyme digestion using normal tendon tissues from three donors. We used the magnetic kit and well-known MSC markers (CD90 or CD105) to isolate MSCs in tendon-derived cells using MACS. Cloning cylinders were used to isolate colony-forming cells having MSC characteristics in tendon-derived cells. Colony-forming unitfibroblast (CFU-F) assay was used to evaluate the self-renewal capacity of cells isolated using the colony picking method or MACS. For comparison of differentiation potentials into osteogenic or adipogenic lineage between two groups, alizarin red S and oil red O staining were performed at 14 days after induction of differentiation in vitro. Results: Flow cytometry results showed that early passage tendon-derived cells expressed CD44 in 99.13%, CD90 in 56.51%, and CD105 in 86.19%. In the CFU-F assay, CD90+ or CD105+ cells isolated with MACS showed larger colony formation in size than cells isolated using the colony picking method. We also observed that CD90+ or CD105+ cells were constantly differentiated into both osteogenic and adipogenic lineages in cells from all donors, whereas cells isolated using the colony picking method were heterogeneous in differentiation potentials to the osteogenic and adipogenic lineages. Conclusion: CD90+ or CD105+ cells isolated using MACS showed superior MSC characteristics in the self-renewal and multi-differentiation capacities compared with cells isolated using the colony picking method. [ABSTRACT FROM AUTHOR]

Details

Language :
Korean
ISSN :
17383757
Volume :
18
Issue :
3
Database :
Academic Search Index
Journal :
Journal of Korean Foot & Ankle Society
Publication Type :
Academic Journal
Accession number :
98177892
Full Text :
https://doi.org/10.14193/jkfas.2014.18.3.100