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Design of Ratiometric Emission Probe with Visible Light Excitation for Determination of Ca2+ in Living Cells.
- Source :
-
Analytical Chemistry . 8/19/2014, Vol. 86 Issue 16, p8025-8030. 6p. - Publication Year :
- 2014
-
Abstract
- An organic salt as a fluorescent probe based on intramolecular charge transfer for Ca2+ determination is developed. Ca2+ can be detected by ratiometric emission at 490 and 594 nm with an excitation wavelength of 405 nm. This probe is highly selective for Ca2+ over other divalent metal cations and displays a large Stokes shift of 189 nm that can avoid interference of the excitation light beam and autofluorescence of biological samples. The dissociation constant for Ca2+ is 2.25 ± 0.47 μM and pertinent to Ca2+ detection in cellular resting and dynamic states. The probe demonstrates its application in monitoring Ca2+ in living cells under confocal microscopic imaging. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 86
- Issue :
- 16
- Database :
- Academic Search Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 98203315
- Full Text :
- https://doi.org/10.1021/ac5022002