Characterization of mustard seeds and paste by DART ionization with time-of-flight mass spectrometry.

Direct analysis in real time (DART) is a novel technique with great potential for rapid screening analysis. The DART ionization method coupled with high-resolution time-of-flight mass spectrometry (TOF-MS) has been used for characterization of mustard seeds and table mustard. The possibility to use DART to analyse glucosinolates was confirmed on determination of sinalbin (4-hydroxybenzyl glucosinolate). The DART-TOF-MS method was optimized and validated. A set of samples of mustard seeds and mustard products was analyzed. High-performance liquid chromatography and DART-TOF-MS were used to determine glucosinolates in mustard seeds and compared. The correlation equation between these methods was DART = 0.797*HPLC + 6.987, R2 = 0.972. The DART technique seems to be a suitable method for evaluation of the quality of mustard seeds and mustard products. Copyright © 2014 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]