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ESTs from the basidiomycete Schizophyllum commune grown on nitrogen-replete and nitrogen-limited media

Authors :
Guettler, S.
Jackson, E.N.
Lucchese, S.A.
Honaas, L.
Green, A.
Hittinger, C.T.
Tian, Y.
Lilly, W.W.
Gathman, A.C.
Source :
Fungal Genetics & Biology. Jul2003, Vol. 39 Issue 2, p191. 8p.
Publication Year :
2003

Abstract

Lambda phage cDNA libraries were constructed using mRNAs from the basidiomycete Schizophyllum commune grown on media with high or low nitrogen concentrations. A total of 440 clones were sequenced, representing 373 distinct transcripts. Of these, 166 showed significant similarity to annotated genes in GenBank. Those that could be tentatively identified using BLAST searches were classified by function using the Gene Ontology (GO) database. Genes with products involved in cell-cycle processes were more frequent in the nitrogen-limited libraries, while genes with products involved in protein biosynthesis were more frequent in the nitrogen-replete library. Overall, clones showed much greater similarity to the one publicly available basidiomycete genome, Phanerochaete chrysosporium, than to any of the ascomycete genomes. [Copyright &y& Elsevier]

Subjects

Subjects :
*BASIDIOMYCETES
*DNA

Details

Language :
English
ISSN :
10871845
Volume :
39
Issue :
2
Database :
Academic Search Index
Journal :
Fungal Genetics & Biology
Publication Type :
Academic Journal
Accession number :
9853408
Full Text :
https://doi.org/10.1016/S1087-1845(03)00017-3