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Increased abundance of cyclooxygenase-2 correlates with vascular endothelial growth factor-A abundance and tumor angiogenesis in gastric cancer
- Source :
-
Cancer Letters . May2003, Vol. 195 Issue 1, p43-51. 9p. - Publication Year :
- 2003
-
Abstract
- To understand the role of cyclooxygenase-2 (COX-2) in gastric cancer, we examined the abundance of COX-2, vascular endothelial growth factor-A (VEGF-A), and CD34 in 45 surgically resected human gastric cancers and paired normal gastric mucosa by immunohistochemical analysis. In addition, the message RNA (mRNA) expression of COX-2 and VEGF-A was evaluated in ten fresh surgically resected human gastric cancers and paired normal gastric mucosas using semi-quantitative reverse transcriptional polymerase chain reaction analysis. Our results confirmed an increased abundance of COX-2 and VEGF-A, and the microvessel density, which was assessed by CD34 abundance, in gastric cancer tissues compared with normal paired mucosa. Abundance of COX-2 and VEGF-A was significantly associated with tumor-node-metastasis (TNM) stage (<f>P<0.05</f>) and lymph node metastasis (<f>P<0.001</f>). In addition, abundance of VEGF-A associates with distance metastasis. A significant correlation was found between COX-2 and VEGF-A abundances (<f>P<0.001</f>). Both abundance of COX-2 and VEGF-A were significantly correlated with microvessel density (<f>P<0.001</f>, respectively). In six of ten cancerous tissues and in one of ten paired normal mucosas, the mRNA expression of COX-2 and VEGF-A was detected in the same specimen. In one other cancerous tissue, only COX-2 mRNA was detected. This study indicates that COX-2 is related to tumor angiogenesis in gastric cancer. VEGF-A might play a main role in the COX-2 angiogenic pathway. [Copyright &y& Elsevier]
- Subjects :
- *CYCLOOXYGENASE 2
*NEOVASCULARIZATION
*ANTIGEN analysis
*CANCER
*COMPARATIVE studies
*GASTRIC mucosa
*GENES
*ISOENZYMES
*RESEARCH methodology
*MEDICAL cooperation
*MEMBRANE proteins
*METASTASIS
*OXIDOREDUCTASES
*POLYMERASE chain reaction
*PROTEINS
*RESEARCH
*RNA
*STOMACH tumors
*EVALUATION research
*VASCULAR endothelial growth factors
*REVERSE transcriptase polymerase chain reaction
*ENDOTHELIAL growth factors
*PATHOLOGIC neovascularization
*METABOLISM
Subjects
Details
- Language :
- English
- ISSN :
- 03043835
- Volume :
- 195
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Cancer Letters
- Publication Type :
- Academic Journal
- Accession number :
- 9857593
- Full Text :
- https://doi.org/10.1016/S0304-3835(03)00153-8