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Gambogic acid induces growth inhibition and differentiation via upregulation of p21waf1/cip1 expression in acute myeloid leukemia cells.
- Source :
-
Journal of Asian Natural Products Research . Oct2014, Vol. 16 Issue 10, p1000-1008. 9p. - Publication Year :
- 2014
-
Abstract
- Gambogic acid (GA) is the major active ingredient of gamboges, a brownish to orange resin product from Garcinia hanburyi tree in Southeast Asia. This compound exhibits anti-cancer effect on solid tumors. In this study, we investigated the effects of GA on the growth and differentiation of acute myeloid leukemia cells by growth-inhibition detection, morphological changes observation, nitroblue tetrazolium reduction, and the expression of the relative cell-surface differentiation markers. The results showed that GA could inhibit cell growth and promote differentiation in U937 and HL-60 cells. In addition, GA upregulated the expression of p21waf1/cip1 in the two cell lines. Finally, downregulating the p21waf1/cip1 expression with small interfering RNA partially blocked GA-induced cell growth inhibition and differentiation. These results of this study revealed that GA may be used as one of the investigational drugs for acute myeloid leukemia. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANALYSIS of variance
*ANTINEOPLASTIC agents
*BIOLOGICAL assay
*CARBOXYLIC acids
*CELL culture
*CELL differentiation
*CELL physiology
*FLOW cytometry
*GENE expression
*GUMS & resins
*MOLECULAR structure
*RESEARCH funding
*TOXICITY testing
*WESTERN immunoblotting
*ACUTE myeloid leukemia
*DATA analysis software
*DESCRIPTIVE statistics
Subjects
Details
- Language :
- English
- ISSN :
- 10286020
- Volume :
- 16
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Journal of Asian Natural Products Research
- Publication Type :
- Academic Journal
- Accession number :
- 98579995
- Full Text :
- https://doi.org/10.1080/10286020.2014.918108