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Radioprotective effects of Antrodia cinnamomea are enhanced on immune cells and inhibited on cancer cells.
- Source :
-
International Journal of Radiation Biology . Oct2014, Vol. 90 Issue 10, p841-852. 12p. - Publication Year :
- 2014
-
Abstract
- Purpose: The radioprotective effects of Antrodia cinnamomea (AC) were investigated for understanding the potential usefulness of AC as an adjunct treatment for reducing radiation side-effects. Materials and methods: In this study, we determined the ability of AC extracts (AC539) to reduce radiation side-effects by analyzing cellular viability in normal mouse spleen immune cells and human cancer cells with different radiosensitivity. We further detected the effect of AC on radiation-induced changes in cytokine- and inflammatory-related gene expressions. Furthermore, apoptosis assay was performed to determine whether AC could inhibit radiation-induced cytotoxicity. Results: We found that an AC dose of 100-150 μg/ml in a time-dependent manner was the most effective in blocking radiation-induced cytotoxicity, in vitro. Radiation-induced cytotoxicity was inhibited in spleen immune cells by 37-56%; however, pretreatment of human colorectal cancer cell line HT-29 with AC did not have any effect on radiation-induced cytotoxicity, while pretreatment of radiosensitive human breast cancer cell lines BT-474 with AC caused a moderate enhancement of radiation-induced damage. Furthermore, AC pretreatment differentially regulated the mRNA expression of several important immunomodulatory genes in response to irradiation in normal and cancer cells. Conclusions: Our data indicate that AC may inhibit important immunoregulatory signaling which could be vital in the avoidance of an over-activated cytotoxic and inflammatory response of the immune system caused by radiation-induced tissue damage. Additionally, AC does not provide a radioprotective effect to tumor cells but instead enhances radiation-induced inflammation and cytotoxicity in cancer. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RADIATION-protective agents
*APOPTOSIS
*PLANT extracts
*CANCER research
Subjects
Details
- Language :
- English
- ISSN :
- 09553002
- Volume :
- 90
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- International Journal of Radiation Biology
- Publication Type :
- Academic Journal
- Accession number :
- 98581339
- Full Text :
- https://doi.org/10.3109/09553002.2014.911989