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FGFR-ERK signaling is an essential component of tissue separation.
- Source :
-
Developmental Biology . Nov2014, Vol. 395 Issue 1, p154-166. 13p. - Publication Year :
- 2014
-
Abstract
- Formation of a constriction and tissue separation between parent and young polyp is a hallmark of the Hydra budding process and controlled by fibroblast growth factor receptor (FGFR) signaling. Appearance of a cluster of cells positive for double phosphorylated ERK (dpERK) at the late separation site indicated that the RAS/MEK/ERK pathway might be a downstream target of the Hydra Kringelchen FGFR. In fact, inhibition of ERK phosphorylation by the MEK inhibitor U0126 reversibly delayed bud detachment and prevented formation of the dpERK-positive cell cluster indicating de novo -phosphorylation of ERK at the late bud base. In functional studies, a dominant-negative Kringelchen FGFR prevented bud detachment as well as appearance of the dpERK-positive cell cluster. Ectopic expression of full length Kringelchen, on the other hand, induced a localized rearrangement of the actin cytoskeleton at sites of constriction, localized ERK-phosphorylation and autotomy of the body column. Our data suggest a model in which (i) the Hydra FGFR targets, via an unknown pathway, the actin cytoskeleton to induce a constriction and (ii) FGFR activates MEK/ERK signaling at the late separation site to allow tissue separation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00121606
- Volume :
- 395
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Developmental Biology
- Publication Type :
- Academic Journal
- Accession number :
- 98847015
- Full Text :
- https://doi.org/10.1016/j.ydbio.2014.08.010