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RNA Tertiary Structure Analysis by 2'-Hydroxyl Molecular Interference.
- Source :
-
Biochemistry . 11/4/2014, Vol. 53 Issue 43, p6825-6833. 9p. - Publication Year :
- 2014
-
Abstract
- We introduce a melded chemical and computational approach for probing and modeling higher-order intramolecular tertiary interactions in RNA 2'-Hydroxyl molecular interference (HMX) identifies nucleotides in highly packed regions of an RNA by exploiting the ability of bulky adducts at the 2'-hydroxyl position to disrupt overall RNA structure. HMX was found to be exceptionally selective for quantitative detection of higher-order and tertiary interactions. When incorporated as experimental constraints in discrete molecular dynamics simulations, HMX information yielded accurate three-dimensional models, emphasizing the power of molecular interference to guide RNA tertiary structure analysis and fold refinement. In the case of a large, multidomain RNA, the Tetrahymena group I intron, HMX identified multiple distinct sets of tertiary structure interaction groups in a single, concise experiment. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00062960
- Volume :
- 53
- Issue :
- 43
- Database :
- Academic Search Index
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 99469432
- Full Text :
- https://doi.org/10.1021/bi501218g