Rab17-mediated recycling endosomes contribute to autophagosome formation in response to Group A S treptococcus invasion.

Autophagy plays a crucial role in host defence by facilitating the degradation of invading bacteria such as Group A S treptococcus ( GAS). GAS-containing autophagosome-like vacuoles ( GcAVs) form when GAS-targeting autophagic membranes entrap invading bacteria. However, the membrane origin and the precise molecular mechanism that underlies GcAV formation remain unclear. In this study, we found that Rab17 mediates the supply of membrane from recycling endosomes ( REs) to GcAVs. We showed that GcAVs contain the RE marker transferrin receptor ( TfR). Colocalization analyses demonstrated that Rab17 colocalized effectively with GcAV. Rab17 and TfR were visible as punctate structures attached to GcAVs and the Rab17-positive dots were recruited to the GAS-capturing membrane. Overexpression of Rab17 increased the TfR-positive GcAV content, whereas expression of the dominant-negative Rab17 form ( Rab17 N132 I) caused a decrease, thereby suggesting the involvement of Rab17 in RE- GcAV fusion. The efficiency of GcAV formation was lower in Rab17 N132 I-overexpressing cells. Furthermore, knockdown of Rabex-5, the upstream activator of Rab17, reduced the GcAV formation efficiency. These results suggest that Rab17 and Rab17-mediated REs are involved in GcAV formation. This newly identified function of Rab17 in supplying membrane from REs to GcAVs demonstrates that RE functions as a primary membrane source during antibacterial autophagy. [ABSTRACT FROM AUTHOR]