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Switchable Enzyme/DNAzyme Cascades by the Reconfiguration of DNA Nanostructures.
- Source :
-
Chemistry - A European Journal . Dec2014, Vol. 20 Issue 49, p16203-16209. 7p. - Publication Year :
- 2014
-
Abstract
- Mimicking cellular transformations and signal transduction pathways by means of biocatalytic cascades proceeding in organized media is a scientific challenge. We describe two DNA machines that enable the 'ON/OFF' switchable activation and deactivation of three-component biocatalytic cascades. One system consists of a reconfigurable DNA tweezers-type structure, whereas in the second system the catalytic cascade proceeds on a switchable DNA clamp scaffold. The three-component catalytic cascades consist of β-galactosidase (β-Gal), glucose oxidase (GOx), and the K+-ion-stabilized hemin-G-quadruplex horseradish peroxidase (HRP)-mimicking DNAzyme. The hemin-G-quadruplex-bridged closed structure of the tweezers or clamp allows the biocatalytic cascades to operate (switched 'ON′′), whereas separation of the hemin-G-quadruplex by means of 18-crown-6-ether opens the tweezers/clamp structures, thus blocking the catalytic cascade (switched 'OFF'). This study is complemented by two-component, switchable biocatalytic cascades composed of GOx and hemin-G-quadruplex assembled on hairpin-bridged DNA tweezers or clamp nanostructures. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BIOCATALYSIS
*DNA
*ENZYMES
*NANOSTRUCTURES
*BIOCHEMISTRY
Subjects
Details
- Language :
- English
- ISSN :
- 09476539
- Volume :
- 20
- Issue :
- 49
- Database :
- Academic Search Index
- Journal :
- Chemistry - A European Journal
- Publication Type :
- Academic Journal
- Accession number :
- 99598359
- Full Text :
- https://doi.org/10.1002/chem.201404122