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Freezing/thawing pretreatment coupled with biological process of thermophilic Geobacillus sp. G1: Acceleration on waste activated sludge hydrolysis and acidification.
- Source :
-
Bioresource Technology . Jan2015, Vol. 175, p509-516. 8p. - Publication Year :
- 2015
-
Abstract
- A novel pretreatment method combining freezing/thawing with Geobacillus sp. G1 was employed to pretreat waste activated sludge (WAS) for enhancing the WAS hydrolysis and subsequent short-chain fatty acids (SCFAs) production. Results showed that freezing/thawing combined with Geobacillus sp. G1 pretreatment achieved the maximal concentrations of soluble protein from 40 ± 6 mg COD/L (non-pretreated) to 1226 ± 24 mg COD/L (pretreated), and accumulated SCFAs concentration increased from 248 ± 81 mg COD/L to 3032 ± 53 mg COD/L. Excitation emission matrix (EEM) fluorescence spectroscopy revealed the highest fluorescence intensity (FI) of protein-like substances, which was the dominant fluorescent organic matters, indicating the synergistic effect of freezing/thawing and Geobacillus sp. G1 pretreatment on organics hydrolysis. High-throughput pyrosequencing analysis investigated that the abundance of bacteria responsible for WAS hydrolysis (such as Clostridium and Caloramator ) and SCFAs production (such as Parabacteroides and Bacterodies ) was greatly enhanced due to the novel pretreatment method used. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09608524
- Volume :
- 175
- Database :
- Academic Search Index
- Journal :
- Bioresource Technology
- Publication Type :
- Academic Journal
- Accession number :
- 99739217
- Full Text :
- https://doi.org/10.1016/j.biortech.2014.10.154