Back to Search Start Over

Data analysis for 16S microbial profiling from different benchtop sequencing platforms.

Authors :
Pylro, Victor S.
Roesch, Luiz Fernando W.
Morais, Daniel K.
Clark, Ian M.
Hirsch, Penny R.
Tótola, Marcos R.
Source :
Journal of Microbiological Methods. Dec2014, Vol. 107, p30-37. 8p.
Publication Year :
2014

Abstract

Progress in microbial ecology is confounded by problems when evaluating results from different sequencing methodologies. Contrary to existing expectations, here we demonstrate that the same biological conclusion is reached using different NGS technologies when stringent sequence quality filtering and accurate clustering algorithms are applied. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01677012
Volume :
107
Database :
Academic Search Index
Journal :
Journal of Microbiological Methods
Publication Type :
Academic Journal
Accession number :
99790591
Full Text :
https://doi.org/10.1016/j.mimet.2014.08.018