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Mechanosensitive Ca2 +-permeable channels in human leukemic cells: Pharmacological and molecular evidence for TRPV2.
- Source :
-
BBA: Biomembranes . Jan2015 Part A, Vol. 1848 Issue 1, p51-59. 9p. - Publication Year :
- 2015
-
Abstract
- Mechanosensitive channels are present in almost every living cell, yet the evidence for their functional presence in T lymphocytes is absent. In this study, by means of the patch-clamp technique in attached and inside-out modes, we have characterized cationic channels, rapidly activated by membrane stretch in Jurkat T lymphoblasts. The half-activation was achieved at a negative pressure of ~ 50 mm Hg. In attached mode, single channel currents displayed an inward rectification and the unitary conductance of ~ 40 pS at zero command voltage. In excised inside-out patches the rectification was transformed to an outward one. Mechanosensitive channels weakly discriminated between mono- and divalent cations (P Ca /P Na ~ 1) and were equally permeable for Ca 2 + and Mg 2 + . Pharmacological analysis showed that the mechanosensitive channels were potently blocked by amiloride (1 mM) and Gd 3 + (10 μM) in a voltage-dependent manner. They were also almost completely blocked by ruthenium red (1 μM) and SKF 96365 (250 μM), inhibitors of transient receptor potential vanilloid 2 ( TRPV2) channels. At the same time, the channels were insensitive to 2-aminoethoxydiphenyl borate (2-APB, 100 μM) or N-(p-amylcinnamoyl)anthranilic acid (ACA, 50 μM), antagonists of transient receptor potential canonical (TRPC) or transient receptor potential melastatin (TRPM) channels, respectively. Human TRPV2 siRNA virtually abolished the stretch-activated current. TRPV2 are channels with multifaceted functions and regulatory mechanisms, with potentially important roles in the lymphocyte Ca 2 + signaling. Implications of their regulation by mechanical stress are discussed in the context of lymphoid cells functions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00052736
- Volume :
- 1848
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- BBA: Biomembranes
- Publication Type :
- Academic Journal
- Accession number :
- 99826135
- Full Text :
- https://doi.org/10.1016/j.bbamem.2014.09.008