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The role of Cu/Zn-SOD and Mn-SOD in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix.

Authors :
Lu, Xia
Wang, Chao
Liu, Baozhong
Source :
Fish & Shellfish Immunology. Jan2015, Vol. 42 Issue 1, p58-65. 8p.
Publication Year :
2015

Abstract

The copper/zinc superoxide dismutase (Cu/Zn-SOD) and manganese superoxide dismutase (Mn-SOD) could effectively eliminate reactive oxygen species (ROS) and maintain the redox balance of immune system. In the present study, the potential synergy of Cu/Zn-SOD and Mn-SOD in immune system was investigated in the clam Meretrix meretrix . The expression of Cu/Zn-SOD mainly distributed in hepatopancreas and that of Mn-SOD was higher in gill of M. meretrix , and their mRNA and protein activity paralleled with each other. In response to H 2 O 2 challenge, Cu/Zn-SOD mRNA showed significantly higher level at 24 h post-challenge and Mn-SOD mRNA was significantly higher at 12 and 24 h post-challenge in the experimental clams than in the control clams ( P < 0.05). After injection with Vibrio-parahaemolyticus -related bacterium (MM21), the Cu/Zn-SOD mRNA was significantly up-regulated at 24 h and 48 h post-injection and Mn-SOD mRNA was significantly higher at 24 h post-injection in MM21-injected clams than in control clams ( P < 0.05), suggesting that both of them might involve in the immune defense to Vibrio challenge. The mRNA expression of Cu/Zn-SOD and Mn-SOD was examined in a Vibrio -resistant population and a control population after MM21 immersion challenge. The increased transcription of Cu/Zn-SOD and Mn-SOD in the resistant population suggested both of them could benefit the immune system to defend against pathogen infection. As expression of Mn-SOD mRNA depended on stimuli and was more easily inducible, its response to H 2 O 2 and Vibrio challenge was earlier than Cu/Zn-SOD. Our study suggested the redox balance might play an important role in M. meretrix to resist pathogen infection. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10504648
Volume :
42
Issue :
1
Database :
Academic Search Index
Journal :
Fish & Shellfish Immunology
Publication Type :
Academic Journal
Accession number :
99831497
Full Text :
https://doi.org/10.1016/j.fsi.2014.10.027