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Crystallization and preliminary crystallographic study of Porcine epidemic diarrhea virus main protease in complex with an inhibitor.
- Source :
-
Acta Crystallographica: Section F, Structural Biology Communications . Dec2014, Vol. 70 Issue 12, p1608-1611. 4p. - Publication Year :
- 2014
-
Abstract
- Porcine epidemic diarrhea virus (PEDV) mainly infects neonatal pigs, resulting in significant morbidity and mortality. Owing to problems such as long periods of virus shedding, existing vaccines cannot provide complete protection from PEDV infection. The PEDV genome encodes two polyprotein precursors required for genome replication and transcription. Each polyprotein undergoes extensive proteolytic processing, resulting in functional subunits. This process is mainly mediated by its genome-encoded main protease, which is an attractive target for antiviral drug design. In this study, the main protease of Porcine epidemic diarrhea virus in complex with a Michael acceptor was crystallized. The complex crystals diffracted to 2.5 Å resolution and belonged to space group R3, with unit-cell parameters a = 175.3, b = 175.3, c = 58.7 Å. Two molecules were identified per asymmetric unit. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CRYSTALLIZATION
*SUMMER diseases
*DIARRHEA
*ISOSPORIASIS
*INTESTINAL diseases
Subjects
Details
- Language :
- English
- ISSN :
- 2053230X
- Volume :
- 70
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Acta Crystallographica: Section F, Structural Biology Communications
- Publication Type :
- Academic Journal
- Accession number :
- 99853199
- Full Text :
- https://doi.org/10.1107/S2053230X14021876