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Degradation of chloronitrobenzenes by a coculture of Pseudomonas putida and a Rhodococcus sp.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 1999 Mar; Vol. 65 (3), pp. 1083-91. - Publication Year :
- 1999
-
Abstract
- A single microorganism able to mineralize chloronitrobenzenes (CNBs) has not been reported, and degradation of CNBs by coculture of two microbial strains was attempted. Pseudomonas putida HS12 was first isolated by analogue enrichment culture using nitrobenzene (NB) as the substrate, and this strain was observed to possess a partial reductive pathway for the degradation of NB. From high-performance liquid chromatography-mass spectrometry and 1H nuclear magnetic resonance analyses, NB-grown cells of P. putida HS12 were found to convert 3- and 4-CNBs to the corresponding 5- and 4-chloro-2-hydroxyacetanilides, respectively, by partial reduction and subsequent acetylation. For the degradation of CNBs, Rhodococcus sp. strain HS51, which degrades 4- and 5-chloro-2-hydroxyacetanilides, was isolated and combined with P. putida HS12 to give a coculture. This coculture was confirmed to mineralize 3- and 4-CNBs in the presence of an additional carbon source. A degradation pathway for 3- and 4-CNBs by the two isolated strains was also proposed.
- Subjects :
- Acetylation
Biodegradation, Environmental
Chromatography, High Pressure Liquid
Coculture Techniques
Magnetic Resonance Spectroscopy
Mass Spectrometry
Oxidation-Reduction
Pseudomonas putida isolation & purification
Rhodococcus isolation & purification
Soil Microbiology
Soil Pollutants metabolism
Water Microbiology
Water Pollutants, Chemical metabolism
Nitrobenzenes metabolism
Pseudomonas putida growth & development
Pseudomonas putida metabolism
Rhodococcus growth & development
Rhodococcus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 65
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 10049867
- Full Text :
- https://doi.org/10.1128/AEM.65.3.1083-1091.1999