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Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors.
- Source :
-
Cell [Cell] 1999 Feb 19; Vol. 96 (4), pp. 495-506. - Publication Year :
- 1999
-
Abstract
- COPI-coated vesicle budding from lipid bilayers whose composition resembles mammalian Golgi membranes requires coatomer, ARF, GTP, and cytoplasmic tails of putative cargo receptors (p24 family proteins) or membrane cargo proteins (containing the KKXX retrieval signal) emanating from the bilayer surface. Liposome-derived COPI-coated vesicles are similar to their native counterparts with respect to diameter, buoyant density, morphology, and the requirement for an elevated temperature for budding. These results suggest that a bivalent interaction of coatomer with membrane-bound ARF[GTP] and with the cytoplasmic tails of cargo or putative cargo receptors is the molecular basis of COPI coat assembly and provide a simple mechanism to couple uptake of cargo to transport vesicle formation.
- Subjects :
- ADP-Ribosylation Factors
Amino Acid Sequence
Animals
Biological Transport physiology
Carrier Proteins metabolism
Cell Compartmentation physiology
Coatomer Protein
Cytoplasm chemistry
Cytoplasm metabolism
Cytoplasmic Granules chemistry
GTP-Binding Proteins metabolism
Guanosine 5'-O-(3-Thiotriphosphate) pharmacology
Guanosine Triphosphate metabolism
Humans
Lipid Bilayers chemistry
Liposomes chemistry
Liposomes metabolism
Membrane Proteins analysis
Membrane Proteins chemistry
Molecular Sequence Data
Peptide Fragments analysis
Peptide Fragments metabolism
Protein Binding drug effects
Protein Binding physiology
Rabbits
Temperature
Cytoplasmic Granules metabolism
Lipid Bilayers metabolism
Membrane Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0092-8674
- Volume :
- 96
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 10052452
- Full Text :
- https://doi.org/10.1016/s0092-8674(00)80654-6