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Estrogens induce apoptosis in mouse peritoneal macrophages.
- Source :
-
Zhongguo yao li xue bao = Acta pharmacologica Sinica [Zhongguo Yao Li Xue Bao] 1997 May; Vol. 18 (3), pp. 267-70. - Publication Year :
- 1997
-
Abstract
- Aim: To study whether estrogen might induce apoptosis in mouse peritoneal macrophages (MPM).<br />Method: The MPM were isolated and incubated in culture medium containing 17-beta-estradiol, estrone, or equal volume of 100% ethanol as control. DNA fragmentation was visualized by agarose gel electrophoresis.<br />Results: 17-beta-Estradiol 0.01-1 mumol.L-1 or estrone 10-20 mumol.L-1 elicited typical morphological apoptosis and DNA fragmentation in a concentration-dependent manner in MPM. Staurosporine (Sta) 0.01 mumol.L-1, cycloheximide (Cyc) 1 mg.L-1, and tamoxifen (Tam) 10 mumol.L-1 inhibited the DNA fragmentation induced by 17-beta-estradiol 1 mumol.L-1 or estrone 20 mumol.L-1.<br />Conclusion: Estradiol and estrone induced apoptosis in MPM.
- Subjects :
- Animals
Cycloheximide pharmacology
Estrogen Antagonists pharmacology
Female
Mice
Protein Kinase C antagonists & inhibitors
Protein Synthesis Inhibitors pharmacology
Staurosporine pharmacology
Tamoxifen pharmacology
Apoptosis drug effects
DNA Fragmentation drug effects
Estradiol pharmacology
Estrone pharmacology
Macrophages, Peritoneal cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0253-9756
- Volume :
- 18
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Zhongguo yao li xue bao = Acta pharmacologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 10072948