[Effect of stimulation of the superior and inferior spermatic nerves on testosterone secretion and testicular blood flow].

The effects of testicular nerves on regulation of androgen secretion were examined by determining testicular blood flow and testosterone in testicular vein at 10 minute interval before, during, and 10, 20, 30 min after stimulation (Pre. Est. Post. 10. Post 20. Post 30 respectively) of superior or inferior spermatic nerves (SSN or ISN) with 25 Hz high (50-70 V) or low (20-25 V) currency. Blood flow and blood samples of the testicular vein were obtained through a cannula in left side under anesthesia of pentobarbital sodium. Under the stimulation of SSN, blood flow in testicular vein decreased by 44.87% in Est with high voltage whereas with low voltage no change appeared in Est but decreased by 27.25% in the period of poststimulation; although with high voltage of stimulation of SSN a transient fall of testosterone (42.01%) was observed in Est, 2.5 times increase of testosterone occurred in the period of poststimulation, meanwhile the testosterone rose by 87.33% in Est of SSN stimulation with low voltage and was further elevated up to 4 times in post-stimulated period. Unlike the stimulation of SSN, there was no influence of stimulating ISN on testosterone with either high or low voltage whereas there was significant increase of blood flow (12.31%) in the period of Est with high voltage then it tended to decrease after stimulation. The present work supported the hypothesis that testicular nerves involve in regulation of androgen secretion in testis and provided a strong evidence for further investigation of nervous regulation of endocrine in testis.