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Structural elucidation of a novel exopolysaccharide produced by a mucoid clinical isolate of Burkholderia cepacia. Characterization of a trisubstituted glucuronic acid residue in a heptasaccharide repeating unit.
- Source :
-
European journal of biochemistry [Eur J Biochem] 1999 Mar; Vol. 260 (2), pp. 373-83. - Publication Year :
- 1999
-
Abstract
- The structure of the exopolysaccharide (EPS) produced by a clinical isolate of Burkholderia cepacia isolated from a patient with fibrocystic lung disease has been investigated. By means of methylation analyses, carboxyl reduction, partial depolymerization by fuming HCl and chemical degradations such as Smith degradation, lithiumethylenediamine degradation and beta-elimination, supported by GC/MS and NMR spectroscopic analyses, the repeat unit of the EPS has been identified and was shown to correspond to the acidic branched heptasaccharide with the following structure: [formula: see text]. This partially acetylated acidic polymer, distinguished by the presence of the less usual D-isomer of rhamnose and of a trisubstituted glucuronic acid residue, could represent the main EPS produced by this bacterial species.
- Subjects :
- Burkholderia cepacia chemistry
Carbohydrate Sequence
Gas Chromatography-Mass Spectrometry
Glucuronic Acid
Humans
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Polysaccharides, Bacterial isolation & purification
Burkholderia Infections microbiology
Burkholderia cepacia metabolism
Glucuronates analysis
Polysaccharides, Bacterial chemistry
Pulmonary Fibrosis microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2956
- Volume :
- 260
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 10095771
- Full Text :
- https://doi.org/10.1046/j.1432-1327.1999.00171.x