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Glutathione-S-transferase activity in malarial parasites.
- Source :
-
Tropical medicine & international health : TM & IH [Trop Med Int Health] 1999 Apr; Vol. 4 (4), pp. 251-4. - Publication Year :
- 1999
-
Abstract
- Glutathione-S-transferase (GST) activity has been detected in rodent (Plasmodium berghei, P. yoelii), simian (P. knowlesi) and human (P. falciparum) malarial parasites, and in different intraerythrocytic stages of P. knowlesi (schizont > ring > trophozoite). In chloroquine-resistant strains of rodent and human malarial parasites GST activity significantly increases compared to sensitive strains. Further, the increase in enzyme activity is directly related to drug pressure of resistant P. berghei. Complete inhibition of chloroquine-sensitive and resistant P. berghei glutathione-S-transferase activities was observed at 2.5 and 5. micrometer concentration of hemin, respectively. An inverse relationship was found between the heme level and enzyme activity of chloroquine-resistant and sensitive P. berghei. Chloroquine, artemisinin, and primaquine noticeably inhibited GST activity in P. knowlesi.
- Subjects :
- Animals
Antimalarials metabolism
Antimalarials pharmacology
Chloroquine metabolism
Chloroquine pharmacology
Cricetinae
Drug Resistance
Heme analysis
Heme physiology
Humans
Macaca mulatta parasitology
Mesocricetus parasitology
Mice parasitology
Plasmodium berghei chemistry
Plasmodium berghei drug effects
Plasmodium berghei growth & development
Plasmodium falciparum chemistry
Plasmodium falciparum drug effects
Plasmodium falciparum growth & development
Plasmodium knowlesi chemistry
Plasmodium knowlesi drug effects
Plasmodium knowlesi growth & development
Plasmodium yoelii chemistry
Plasmodium yoelii drug effects
Plasmodium yoelii growth & development
Protoporphyrins analysis
Protoporphyrins physiology
Glutathione Transferase physiology
Plasmodium berghei enzymology
Plasmodium falciparum enzymology
Plasmodium knowlesi enzymology
Plasmodium yoelii enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1360-2276
- Volume :
- 4
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Tropical medicine & international health : TM & IH
- Publication Type :
- Academic Journal
- Accession number :
- 10320651
- Full Text :
- https://doi.org/10.1046/j.1365-3156.1999.00387.x