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Identification of markers for precursor and leydig cell differentiation in the adult rat testis following ethane dimethyl sulphonate administration.
- Source :
-
Biology of reproduction [Biol Reprod] 1999 Jun; Vol. 60 (6), pp. 1437-45. - Publication Year :
- 1999
-
Abstract
- Administration of ethane dimethane sulphonate (EDS) to adult rats results in the destruction of all Leydig cells, followed by a complete regeneration. We investigated this regeneration process in more detail, using different markers for precursor and developing Leydig cells: the LH receptor, 3beta-hydroxysteroid dehydrogenase (3beta-HSD), transforming growth factor alpha (TGFalpha), and a new marker for Leydig cell maturation, relaxin-like factor (RLF). LH receptor immunoreactivity was found in Leydig cell-depleted testes at 3 and 8 days after EDS administration. The positive (precursor) cells had a mesenchymal-like morphology. The number of LH receptor-positive cells 8 days after EDS administration was 15 +/- 4 per 500 Sertoli cell nuclei. Fifteen days after EDS administration, the first new Leydig cells could be observed. These cells stained positively with both the antibodies against the LH receptor and 3beta-HSD, while some cells also stained positively for TGFalpha. After EDS administration, RLF mRNA disappeared from the testis and reappeared again at the time of the appearance of the first Leydig cells. Concomitant with the increase in the number of Leydig cells, the number of RLF-expressing cells increased. The observations of the present study give further support to the hypothesis that Leydig cell development in the prepubertal testis, and in the adult testis following EDS administration, takes place along the same cell lineage and suggest, therefore, that the adult EDS-treated rat can serve as a model for studying the adult-type Leydig cell development that normally occurs in the prepubertal rat testis.
- Subjects :
- 3-Hydroxysteroid Dehydrogenases analysis
Animals
Cell Count
Immunohistochemistry
Insulin
Leydig Cells cytology
Leydig Cells drug effects
Male
Proteins analysis
Proteins genetics
RNA, Messenger analysis
Rats
Rats, Wistar
Receptors, LH analysis
Stem Cells cytology
Testis cytology
Testis drug effects
Transforming Growth Factor alpha analysis
Biomarkers analysis
Cell Differentiation
Leydig Cells chemistry
Mesylates pharmacology
Stem Cells chemistry
Testis chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3363
- Volume :
- 60
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biology of reproduction
- Publication Type :
- Academic Journal
- Accession number :
- 10330103
- Full Text :
- https://doi.org/10.1095/biolreprod60.6.1437