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Role of glucocorticoids in the maturation of renal cortical Na+-K+-ATPase during fetal life in sheep.
- Source :
-
The American journal of physiology [Am J Physiol] 1999 Jun; Vol. 276 (6), pp. R1825-32. - Publication Year :
- 1999
-
Abstract
- Glucocorticoid levels increase greatly at the time of birth in humans and sheep, coinciding with an increased ability of the kidney to reabsorb sodium. Cortisol induces proximal tubule apical membrane Na+/H+ exchanger maturation in near-term fetal sheep. Proximal tubule salt transport is ultimately dependent on Na+ pump activity, so we studied the effects of cortisol treatment on renal cortical Na+-K+-ATPase. We first looked at six 140 day gestation fetal sheep (term is 145) and compared their renal cortical Na+-K+-ATPase to that of six 1-day-old lambs. Na+-K+-ATPase activity increased 80% after birth. Then nine pairs of twin fetal sheep were chronically instrumented at 127 days gestation. After 72 h recovery, one twin was given a 48-h continuous intraperitoneal infusion of cortisol. Both twins were then killed, and their renal cortices were studied. Na+-K+-ATPase activity increased 122% with cortisol treatment; activity equaled that of 1-day-old lambs. Protein abundance of the alpha1-subunit of the Na+-K+-ATPase increased 19%; the beta1-subunit increased 39% with cortisol treatment. mRNA abundance of the alpha1-subunit increased 58%; the beta1-subunit increased 72%. These results indicate that cortisol matures Na+-K+-ATPase activity.
- Subjects :
- Animals
Animals, Newborn metabolism
Animals, Newborn physiology
Hydrocortisone pharmacology
Immunoblotting
Kidney Cortex enzymology
RNA, Messenger metabolism
Sheep embryology
Sodium-Potassium-Exchanging ATPase genetics
Fetus metabolism
Glucocorticoids physiology
Kidney Cortex embryology
Sodium-Potassium-Exchanging ATPase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 276
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 10362766
- Full Text :
- https://doi.org/10.1152/ajpregu.1999.276.6.R1825